CHROMATOGRAPHIES

At NanoBusiness, you will find alternatives in Chromatography to accurately purify, identify and quantify chemical compounds.

As it is a broad method, it has several classifications and analysis options, among which we offer:

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Indicated to separate and quantify the individual components of a mixture, this technique has high resolution and detectability conditions, being an advanced type of Liquid Chromatography (LC). Due to its great analytical potential, it has been widely used for:

  • Quantification and purification of active ingredients;
  • Impurity identification;
  • Determination of composition or formulation;
  • Stability and degradation studies of a product.

With HPLC, the separation can occur by partition, adsorption, ion exchange, exclusion by size or by difference in affinity, depending on the composition of the column and the mixture to be separated.

Among the available detectors, we find the UV (UV-visible) e DAD (diode array) detectors, in which is possible to analyze compounds with chromophores, high sensitivity and the has the possibility of gradient elution; the RID (Refrative Index) detector, known as the universal detector because it has a response to any kind of sample; and the ELSD (Evaporative Light Scattering) detector, capable of detecting compounds that do not have chromophores. [[top]

HPLC COUPLED TO MASS SPECTROMETRY (HPLC-MS)

The HPLC-MS system combines the HPLC and MS (Mass Spectrometry) techniques in a process in which the liquid chromatography method separates the compounds, and then a mass detector analyzes them. This combination reduces the experimental error and improves the results accuracy, being useful in analyses involving a large number of compounds.

In the pharmaceutical industry, the method is recommended for the Identification of impurities and degradation products in accordance with RDC (ANVISA Board Resolution) No. 53. [[top]

GAS CHROMATOGRAPHY (GC)

Gas chromatography (GC) is indicated for the separation and analysis of volatile substances in complex matrices, in which the main separation method is by the interaction of the components with the stationary phase.

Technically, the sample goes through an injection system where the liquid samples are vaporized and then dragged through the chromatographic column by an inert gas (usually helium, hydrogen or argon).

It can be applied in purity tests, separation of components in a mixture, identification or preparation of compounds, and others. [[top]

GAS CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY (GC-MS)

The combination of gas chromatography and mass spectrometry provides more accurate analyses and reduces the probability of error, because different molecules are unlikely to behave the same way in a gas chromatograph and in a mass spectrometer.

In the pharmaceutical industry, the method is recommended for the identification of impurities and residual solvents. [top]